The genotypes of most of the animal fecal isolates were unique and occupied a common space in the negative PC1 area forming a separate cluster. The most frequent genotypes were grouped in the positive zone of both the principal coordinates (PC1 and PC2). Principal coordinate analysis (PCoA) showed limited clustering of genotypes based on the sampling sites. These sites also exhibited high multiple antibiotic resistance (MAR) indexes (above 0.25) suggesting high risk of contamination. The sampling sites under the influence of high anthropogenic activities showed an increase in number of unique genotype isolates. coli isolates was highly diverse at all the sampling sites across Yamuna River except at its entry site in Delhi. The frequency of genotypes ranged from 0.013 to 0.065. Combination of ERIC types (E1–E32) and (GTG) 5 types (G1–G46) generated 77 genotypes. High genetic diversity was observed by both the methods however, (GTG) 5 typing showed higher discriminating potential. coli isolates belonging to different serotypes, phylogroups, and multi-drug resistance patterns were analyzed. ERIC-PCR and (GTG) 5-PCR and compared with the MDR animal fecal isolates. coli isolated from different sites of Yamuna River was analyzed using repetitive element PCR (rep-PCR) methods viz. Genotypic diversity among multi-drug-resistant (MDR) aquatic E.
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